Carbon monoxide in blood: an improved microliter blood-sample collection system, with rapid analysis by gas chromatography.
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چکیده
منابع مشابه
Carbon monoxide in blood: an improved microliter blood-sample collection system, with rapid analysis by gas chromatography.
We examined the sensitive assay for carboxyhemoglobin based on reaction with K3Fe(CN)6 and gas chromatography of the liberated CO. Our improvements included increased baseline stability, shorter analysis time, and simpler standardization. EDTA-containing Vacutainer Tubes (lavender-stoppered) increase the carboxyhemoglobin content of blood stored in them. The carboxyhemoglobin content of blood s...
متن کاملDetermination of carbon monoxide in blood by head space analysis.
A method for the determining the amount of carbon monoxide (CO) in blood is described. CO bound to hemoglobin is released with potassium ferricyanide into a known volume of head space of a disposable syringe. Vapor in the head space is withdrawn with a gastight microcyringe and injected into the gas chromatograph. CO dissolved in saturated blood is also measured by head space analysis to ascert...
متن کاملRapid, accurate technique for determination of carbon monoxide in blood.
We report a chromatographic technique in which a vortex method of gas extraction and a thermal conductivity detector are used to measure carbon monoxide in blood. This method has the advantages of a small sample volume (100 l), a rapid analysis time (3 mm), ease of operation, and a high correlation with results of the standard gasometric method over the entire possible range (r = 0.999). The ac...
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Head-Space Gas Chromatography-Mass Spectrometry (HS-GC/MS) was used to determine blood carboxyhemoglobin (COHb%), especially in cases involving blood decomposition or death, when blood extraction is impossible. Until now, the problem eluded complete solution using forensic toxicology. Therefore a method was developed using Headspace Gas Chromatography-Mass Spectrometry (HSGC/MS), which should e...
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NanoSystems Biology Cancer Center, Kavli Nanoscience Institute, Division of Chemistry and Chemical Engineering, California Institute of Technology, MC 127-72, 1200 E. California Blvd., Pasadena, California, 91125 USA. Instititue for Systems Biology, 1441 North 34 St., Seattle, Washington, 98103-8904 USA. These authors contributed equally to this work. Correspondence should be addressed to J.R.H...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1984
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/30.8.1382